Objectives

This platform is at the service of studies conducted on fungal species that interact with Mediterranean and tropical plant species considered by the research teams of the Agap unit and their partners. It also hosts the yeast cultures used as vectors in the methodologies of molecular studies conducted on these plants.
The mycology platform is a secure environment that enables all stages of these studies involving the manipulation of live fungal isolates to be carried out.

Know-how, tools and resources

The Plateau has facilities, apparatus and equipment to carry out a number of works on fungal species.
The various activities offered are as follows:

•     Isolation and identification of strains for pure culture,
•     Maintenance of stump collections,
•     Determination of enzymatic activity,
•     Isolation and qualitative and quantitative controls of DNA,
•     Adjustment of PCR conditions of specific markers,
•     Yeast transformation for the creation of double hybrids.

To carry out these activities, 7 work stations are available:

•     Weighing, pH, preparation of solutions and media,
•     Handling in a sterile environment,
•     Sample sorting and microscopic observations,
•     Enzymology,
•     DNA extraction,
•     PCR tuning,
•     Double yeast hybrids.

The plateau also has a collection of isolates of various phytopathogenic and lignivorous fungal species, in culture or in the form of dehydrated sporophores. This collection is available for studies on these species.
Finally, the plateau has an expertise on the basidiomycete fungal model Ganoderma boninense. It includes a large database of genotypes, a reference genome, expression data, knowledge on life traits, and is based on a large collection of cultured or dehydrated samples.